Brief introduction to the index status of carbon dioxide concentration in carbon dioxide incubator

Issue date:2022/03/08    Views：67    Classification：Industry information

Jiangsu Tianling carbon dioxide incubator is a device for in vitro culture of cells / tissues by simulating the formation of a growth environment similar to cells / tissues in organisms in the incubator, such as stable temperature (37 ° C), stable CO2 level (5%), constant pH (pH: 7.2-7.4), and high relative saturation humidity (95%). It is widely used in cell and tissue culture and the culture of some special microorganisms. It is commonly used in the research fields of cell dynamics, the collection of mammalian cell secretions, the carcinogenic or toxicological effects of various physical and chemical factors, the research and production of antigens, the production of antibodies by cultivating hybridoma cells, in vitro fertilization (IVF), stem cells, tissue engineering, drug screening and so on.

Users have two basic requirements for carbon dioxide incubators. One is that carbon dioxide incubators can provide stable control of temperature, carbon dioxide concentration and humidity, so as to facilitate the progress of their research work; Second, the carbon dioxide incubator is required to effectively prevent microbial pollution in the incubator, and regularly eliminate pollution, so as to protect research results and prevent sample loss.

1、 Heating mode

Air jacket heating and water jacket heating, both heating systems are and reliable, and they have their own advantages and disadvantages. Water jacket heating is to maintain a constant temperature by surrounding the internal box with an independent water jacket layer. Its advantages: water is a good insulating material. When power failure occurs, the water jacket system can maintain the accuracy and stability of the temperature in the incubator for a long time, which is conducive to the selection of users with unstable experimental environment (such as power limit or frequent power failure). Air jacket heating is to directly heat the inner box body through the heaters throughout the air jacket layer of the box body, also known as six side direct heating. Compared with the water jacket type, the air jacket type has the characteristics of faster heating and faster temperature recovery than the water jacket type incubator, which is especially conducive to short-term culture and culture requiring frequent opening and closing of the door. In addition, for users, the air jacket design is simpler than the water jacket design (the water jacket needs to add water, empty and clean the water tank, and often monitor the operation of the water tank, as well as potential pollution hazards).

2、 Microprocessor control system

The micro processing control system is an operating system to maintain the steady state of temperature, humidity and CO2 concentration in the incubator. The application of microprocessor control system and other functional accessories (such as high and low temperature automatic adjustment and alarm device, CO2 alarm device, password protection setting, etc.) makes the operation and control of carbon dioxide incubator very simple. For example, the PID microprocessor touch screen control system of LEEC can strictly control the concentration of gas and reduce its loss to a very low level, so as to ensure that the culture environment is constant, and can ensure the temperature in the box during the long-term culture process. It has liquid crystal display, graphical process monitoring, dry pre event recording, etc. In addition, the alarm system is also indispensable. It can let you know the situation of the incubator in time and respond, so as to minimize the loss and ensure the continuity of the experiment. Some incubators have sound / light alarm devices. When the temperature changes to ± 0.5 ℃, or the CO2 concentration changes to ± 5%, it will automatically alarm; Some have CO2 concentration abnormal alarm display function; Some have low-voltage and power-off alarm functions. These devices are designed to facilitate users and reduce the tedious and boring experimental process.

3、 Control of pollutants

Pollution is a major factor leading to the failure of cell culture. Jiangsu Tianling Instrument Co., Ltd. has designed a variety of different devices to reduce and prevent the occurrence of pollution. The main way is to minimize the areas and surfaces where microorganisms can grow, and to effectively prevent the occurrence of pollution in combination with automatic pollution removal devices. For example, in view of the fact that CO2 incubators are sometimes accompanied by mold growth during use, some companies have developed and designed CO2 incubators with UV disinfection function in order to ensure that the incubators are free from pollution and ensure the biological cleanliness of the instrument box; In addition, HEPA high-efficiency filter designed by the company can filter the air in the incubator and remove 99.97% of particles above 0.3um; In addition, the automatic high-temperature hot air sterilization device can make the temperature in the box reach a high temperature (such as 200 ℃), so as to kill all polluting microorganisms, even spores and other high-temperature resistant microorganisms. These devices are more safe for cell culture.

4、 Carbon dioxide concentration control optional carbon dioxide transmitter model: k33-icb-20%

Infrared sensor (IR) or thermal conductivity sensor (TC) for measurement. Both sensors have their own advantages and disadvantages. The working principle of the thermal conductivity sensor for monitoring CO2 concentration is based on the continuous measurement of the thermal conductivity of the air in the cavity. The low thermal conductivity of the input CO2 gas will change the thermal conductivity of the air in the cavity, which will produce an electrical signal directly proportional to the CO2 concentration. One disadvantage of TC control system is that the change of temperature and relative humidity in the box will affect the accuracy of the sensor. When the box door is frequently opened, not only CO2 concentration, temperature and relative humidity will also fluctuate greatly, thus affecting the accuracy of TC sensor. This control system is not applicable when the required culture conditions and the door of the incubator are opened frequently. Infrared sensor (IR) it detects CO2 level through an optical sensor. The IR system includes an infrared transmitter and a sensor. When the CO2 in the tank absorbs part of the infrared emitted by the transmitter, the sensor can detect the reduction of infrared, and the amount of absorbed infrared corresponds to the level of CO2 in the tank, so the concentration of CO2 in the tank can be obtained. Because the IR system determines the CO2 concentration in the box through infrared ray reduction, and the particles in the box can reflect or partially absorb infrared ray, the IR system is more sensitive to the number of particles in the box. The IR sensor is suitable for the incubator with HEPA high-efficiency air filter at the air inlet.

5、 Relative humidity optional temperature and humidity transmitter H2 series

Humidity in the box is a very important but often neglected factor for cultivation. Maintain a sufficient humidity level and have a fast enough humidity recovery speed (such as after opening and closing the door) to ensure that the culture will not fail due to excessive drying. At present, most carbon dioxide incubators produce moisture through the evaporation of humidifying plates (the relative humidity level produced can reach about 95%, but the humidity recovery speed is very slow after opening the door). Try to choose the incubator with large humidity evaporation area, because the larger the humidity evaporation area, the easier it is to reach the relative saturation humidity, and the shorter the time of humidity recovery after opening and closing the door.